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ATCC -30502™
  • 平台编号:bio-48368
  • 规格:frozen
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ATCC® Number:30502™
Organism: Leishmania donovani (Laveran and Mesnil) Ross
Designations: ET
Isolation: human, Burma pre-1959
Depositors: R Herman
History: ATCC<<--R Herman<<--L. Stauber <<--- L.G. Goodwin
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, golden hamster Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCCmedium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Amastigotes. polyamine function [23571]
References: 4623: Stauber LA. Characterization of strains of Leishmania donovani. Exp. Parasitol. 18: 1-11, 1966. PubMed: 5909670 23571: Bacchi CJ, et al. Polyamine function in trypanosomatids: activation of cytoplasmic alpha-glycerophosphate dehydrogenase by cationic trypanocides. Adv. Polyamine Res. 2: 129-138, 1978.
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