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ATCC -VR-2029™
  • 平台编号:bio-49419
  • 规格:frozen
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ATCC® Number:VR-2029™
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus
Strain: vP-6
Depositors: E Paoletti
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : HeLa cells (ATCCCCL-2); CV-1 cells (ATCCCCL-70)
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : A recombinant virus consisting of a TK -vaccinia (VTK-11) with an HSV-TK insert which is expressed. Two copies of the herpes virus BamHI fragment coding for TK were cloned (head to tail) into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-11 by in vivo recombination. [13123]
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520
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