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MH1C1
  • 平台编号:bio-68186
  • 规格:0.2ml
  • 拉丁属名:
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运输方式:冻存运输
细胞形态:上皮样
ATCC Number:CCL-144?
器官来源:肝
相关疾病:肝癌
是否是肿瘤细胞:0
物种来源:大鼠
生长状态:贴壁生长
数量:大量
规格:0.2ml Designations: MH1C1
Depositors: ?AH Tashjian, U Richardson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial


Source: Organ: liver
Disease: hepatoma
Cellular Products:rat serum albumin; complement components (especially C9)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Comments:Cells conjugate and metabolize testosterone and bilirubin.
Propagation: ATCC complete growth medium: Hams's F12K medium with 2.0 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%

Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 1026: Richardson UI, et al. Establishment of a clonal strain of hepatoma cells which secrete albumin. J. Cell Biol. 40: 236-247, 1969. PubMed: 4177659
1028: Rommel FA, et al. Synthesis of the ninth component of complement by a clonal strain of rat hepatoma cells. J. Immunol. 105: 396-403, 1970. PubMed: 4194308
26006: Tashjian AH Jr., et al. Multiple differentiated functions in an unusual clonal strain of hepatoma cells. In Vitro 6: 32-45, 1970. PubMed: 4399803
26009: . . Science 170: 533, 1970.
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