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Hs 832(C).T [Hs832.Tc]
  • 平台编号:bio-68350
  • 规格:0.1ml
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组织来源:cyst, benign
细胞形态:成纤维样
器官来源:卵巢
ATCC Number:CRL-7566?
相关疾病:其他疾病
生长状态:贴壁生长
是否是肿瘤细胞:0
物种来源:人
数量:大量
运输方式:冻存运输
规格:0.1ml Designations: Hs 832(C).T [Hs832.Tc]
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: ovary
Tissue: cyst, benign
Disease: endometriosis
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: United States
Isolation date: 1974
Gender: female
Ethnicity: Caucasian
Comments:The line was established from a benign ovarian cyst taken from a patient with endometriosis. The cells were isolated from both the inner and outer surfaces of the cyst. The line senesces after passage 28.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Preservation: Freeze medium: 50% FBS, 40% Growth Medium, 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 72 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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