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Ray Hot
  • 平台编号:bio-68758
  • 规格:1mg
  • 拉丁属名:
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运输方式:冻存运输
器官来源:皮肤
生长状态:贴壁生长
是否是肿瘤细胞:0
物种来源:人
ATCC Number:CRL-1226?
相关疾病:其他疾病
细胞形态:成纤维样
年限:23 years
数量:大量
规格:1mg Designations: Ray Hot
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: skin
Disease: Ehlers-Danlos syndrome, type III
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 23 years
Gender: male
Comments:The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227 ) who was affected with the same condition.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
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