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TR 310
  • 平台编号:bio-68922
  • 规格:0.5mg
  • 拉丁属名:
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生长状态:悬浮生长
ATCC Number:HB-219?
运输方式:冻存运输
数量:大量
是否是肿瘤细胞:0
物种来源:大鼠
细胞形态:淋巴样
免疫类型:IgG2b
规格:0.5mg Designations: TR 310
Depositors: ?IL Weissman
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse T cell receptor
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the Tk1 murine T cell lymphoma.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a deteminant on the mouse T cell antigen receptor.
It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
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