Clostridium perfringens (Veillon and Zuber) Hauduroy et al. 拉丁名
ATCC 19574 编号
Toxinotype A 毒型A
Product category
Bacteria
Product type
Anaerobe
Strain designation 别名 43F4
Type strain 模式菌株 No
Isolation 分离源 source
Bile
Geographical isolation
United States; Maryland; Baltimore
Applications
Agricultural research
Enteric disease research
Infectious disease research
Product format 提供形式 Freeze-dried
Storage conditions 保藏条件 2°C to 8°C
Specific applications
Enteric Research
Preceptrol
No
Genotype
cpa, cpe, cpb2, pfoA
Toxigenic
Yes
Toxin genes
cpa (Alpha toxin) positive
cpb2 (Beta2 toxin) positive
cpe (Enterotoxin) positive
etx (Epsilon toxin) negative
pfoA (Perfringolysin O) positive
Comments
Toxinotype A
Hydrolyzes bile acid conjugates
The presence of cpa, cpe, cpb2, and pfoA genes was confirmed by PCR.
This strain does not produce epsilon toxin
Medium 培养基 ATCC Medium 2107: Modified Reinforced Clostridial
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature 培养温度 37°C
Atmosphere 需氧情况 Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth.
Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth.
Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
Deposited as
Clostridium perfringens (Veillon and Zuber) Hauduroy et al.
Depositors
JF Reback
Type of isolate
Human