Myceliophthorathermophila(Apinis)vanOorschot
42464™
Anampoulecontainingviablecells(mayincludesporesandmycelia)suspendedincryoprotectant.
Productcategory
Fungi
Typestrain
No
Genomesequencedstrain
Yes
Isolationsource
Soil
Productformat
Freeze-dried
Storageconditions
2°Cto8°C
General
Specificapplications
Producescellobiosedehydrogenase
Producesglucosidase,beta;acidGlucosidase,beta,acid,glucocerebrosidase
Preceptrol
No
Characteristics
Comments
Effectofbeta-glucosidaseinhibitorsonsynthesisofcellulaseandbeta-glucosidase
Isolationofprotoplasts
Genomesequencingstrain(theJointGenomeInstituteattheDepartmentofEnergy,USA).
Handlinginformation
Medium
ATCCMedium350:EmersonYpSsagar
ATCCMedium336:Potatodextroseagar(PDA)
ATCCMedium200:YMagarorYMbroth
Temperature
37-45°C
Atmosphere
Aerobic
Handlingprocedure
Forfreeze-dry(lyophilized)ampoules:
Openanampouleaccordingtoenclosedinstructions.
Fromasingletesttubeofsteriledistilledwater(5to6mL),withdrawapproximately0.5to1.0mLwithasterilepipetteandapplydirectlytothepellet.Stirtoformasuspension.
Asepticallytransferthesuspensionbackintothetesttubeofsteriledistilledwater.
Letthetesttubesitatroomtemperature(25°C)undisturbedforatleast2hours;longer(e.g.,overnight)rehydrationmightincreaseviabilityofsomefungi..
Mixthesuspensionwell.Useseveraldrops(ormakedilutionsifdesired)toinoculaterecommendedsolidorliquidmedium.Includeacontrolthatreceivesnoinoculum.
Incubatetheinoculumatthepropagationconditionsrecommended.
Inspectforgrowthoftheinoculum/strainregularly.Thesignofviabilityisnoticeabletypicallyafter2-4daysofincubation.However,thetimenecessaryforsignificantgrowthwillvaryfromstraintostrain.
Handlingnotes
Overnightrehydrationofastrainvialisrecommendedasitproducedmorecolonies.Freshlymadeandmoistmediumhasbeenshowntofacilitatetherevivabilityofpreservedsamples.Thepropertyofgrowthat45°Cisbestobservedwhencultureistransferredfromthosealreadygrownat30°Cto37°C,insteaddirectlyfromrehydratedvials.Incubationat45°Cneedsanincubatorthateitherhashumiditycontrolorhasapanofsterilewatertokeeptheincubatorfromdrying.
Additional,updatedinformationonthisproductmaybeavailableontheATCC®websiteatwww.atcc.org.
History
Depositedas
SporotrichumthermophileApinis
Depositors
TBarnes
Chainofcustody
ATCC<--TBarnes
Typeofisolate
Environmental
Legaldisclaimers
Intendeduse
Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse.
Warranty
Theproductisprovided'ASIS'andtheviabilityofATCC®productsiswarrantedfor30daysfromthedateofshipment,providedthatthecustomerhasstoredandhandledtheproductaccordingtotheinformationincludedontheproductinformationsheet,website,andCertificateofAnalysis.Forlivingcultures,ATCCliststhemediaformulationandreagentsthathavebeenfoundtobeeffectivefortheproduct.Whileotherunspecifiedmediaandreagentsmayalsoproducesatisfactoryresults,achangeintheATCCand/ordepositor-recommendedprotocolsmayaffecttherecovery,growth,and/orfunctionoftheproduct.Ifanalternativemediumformulationorreagentisused,theATCCwarrantyforviabilityisnolongervalid.Exceptasexpresslysetforthherein,nootherwarrantiesofanykindareprovided,expressorimplied,including,butnotlimitedto,anyimpliedwarrantiesofmerchantability,fitnessforaparticularpurpose,manufactureaccordingtocGMPstandards,typicality,safety,accuracy,and/ornoninfringement.
Disclaimers
Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse.AnyproposedcommercialuseisprohibitedwithoutalicensefromATCC.
WhileATCCusesreasonableeffortstoincludeaccurateandup-to-dateinformationonthisproductsheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureandpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeenconfirmedtobeaccurateorcompleteandthecustomerbearsthesoleresponsibilityofconfirmingtheaccuracyandcompletenessofanysuchinformation.
Thisproductissentontheconditionthatthecustomerisresponsibleforandassumesallriskandresponsibilityinconnectionwiththereceipt,handling,storage,disposal,anduseoftheATCCproductincludingwithoutlimitationtakingallappropriatesafetyandhandlingprecautionstominimizehealthorenvironmentalrisk.Asaconditionofreceivingthematerial,thecustomeragreesthatanyactivityundertakenwiththeATCCproductandanyprogenyormodificationswillbeconductedincompliancewithallapplicablelaws,regulations,andguidelines.Thisproductisprovided'ASIS'withnorepresentationsorwarrantieswhatsoeverexceptasexpresslysetforthhereinandinnoeventshallATCC,itsparents,subsidiaries,directors,officers,agents,employees,assigns,successors,andaffiliatesbeliableforindirect,special,incidental,orconsequentialdamagesofanykindinconnectionwithorarisingoutofthecustomer'suseoftheproduct.Whilereasonableeffortismadetoensureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfromthemisidentificationormisrepresentationofsuchmaterials.