Dictyostelium discoideum Raper 拉丁名
ATCC 28368 编号
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation 别名 Ax-3
Type strain 模式菌株 No
Product format 提供形式 Freeze-dried
Storage conditions 保藏温度 2°C to 8°C
Specific applications
produces calmodulin
transformation host
hydrolysis of a phosphonate ester
Preceptrol
No
Comments
Actin binding to plasma membrane
Thymidine kinase activity
Part of the Eumycetozoan Project
This strain may grow in axenic medium or with Klebsiella aerogens, but ATCC maintains this strain with a bacterial food source.
Medium 培养基 ATCC Medium 617: Bg-11 for marine blue-green algae
ATCC Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Temperature 培养温度 20-25°C
Atmosphere 需氧情况 Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use 1.0 mL to inoculate recommended freshly-made and wetted solid medium and freshly-made liquid medium.
Agar plates should be overlaid with 2 mL of a liquid medium, either #671 or #1034 (with 10% fetal bovine serum included).
Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 2-3 days of incubation.
However, the time necessary for significant growth will vary.
Subculture from stationary liquid cultures after one week.
Sequenced data
No DNA sequencing was performed in house on this product.
Deposited as
Dictyostelium discoideum Raper
Depositors
WF Loomis
Chain of custody
ATCC <-- WF Loomis
Special collection
Eumycetozoan Project
Cross references 参考文献 GenBank D12883 gene coding for gp138
GenBank D12884 gene coding for gp138