Rhodobaca bogoriensis Milford et al. 拉丁名
700920 编号
Product category
Bacteria
Product type
Extremophile
Strain designation 别名 LBB1
Type strain 模式菌株 Yes
Isolation 分离源 source
Lake Bogoria
Geographical isolation
Kenya
Product format 提供形式 Freeze-dried
Specific applications
Biotechnology
Preceptrol
No
Extremophile type
Alkaliphile
Phototroph
Medium 培养基 ATCC Medium 2193: RV5 medium
Temperature 培养温度 35°C
Atmosphere 需氧情况 Anaerobic
Incubation
Under light
Handling procedure
1. Put 6 to 8 ml of Medium #2193 into a 13x100 mm screw cap test tube (small). Add cysteine (3.0% stock concentration, 2 ml/100 ml medium) and then fill the test tube to capacity with Medium #2193. Seal the test tube with a screw cap.
2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
3. Open the freezedried vial according to enclosed instructions.
4. Aseptically take 0.5 ml of the pre-reduced medium and rehydrate the pellet.
5. Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity). One or two drops can be streaked out onto #2193 agar plates. This culture grows aerobically in the dark.
6. Incubate the culture at 35oC under a tungsten lamp.
7. Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (1020%) the medium does not need to be pre-reduced.
8. When examined microscopically, the cells are motile rods that occur singly, in pairs and short chainsDeposited as
Rhodobaca bogoriensis Milford et al.
Depositors
MT Madigan
Type of isolate
Environmental
Year of origin
1998
Cross references
GenBank AF248638 16S rRNA sequence