Product category
Bacteria
Strain designation
BB536
Type strain
No
Genome sequenced strain
Yes
Isolation source
Feces of healthy female baby; isolated June 1969
Product format
Freeze-dried
Storage conditions
2°C to 8°C
Specific applications
Probiotic strain
Preceptrol
No
Handling information
Medium
ATCC Medium 1490: Modified chopped meat medium
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1490 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1490 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a brucella blood plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 to 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
Culture may be grown in either 80% N2-10% CO2-10% H2 or 80% N2-20% CO2.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
Verification method
Whole-genome Sequencing
History
Deposited as
Bifidobacterium longum Reuter
Depositors
T Yaeshima, S Shimamura
Chain of custody
T Yaeshima, S Shimamura
Type of isolate
Human
Year of origin
1969
Patient age
infant
Patient gender
Female
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.