Escherichia coli (Migula) Castellani and Chalmers 拉丁名
49980 编号
Product categoryBacteria
Classification Enterobacteriaceae, EscherichiaStrain designationNCIMB 11187 [WP2]Type strainNoIsolation sourceMutant derived from strain B/RProduct formatFreeze-dried
Specific applications
Testing mutagens
Preceptrol No
Genotypetrp-Comments
Mutation induced by freeze-drying
Mutation induced by heliotrine and monocrotaline
Mutation induced by the fungicide captan
Ultraviolet dose-mutation relationships
Medium 培养基
ATCC Medium 3: Nutrient agar or nutrient broth
Temperature 培养温度
37°C
Handling procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
Handling notes
On #3 plates, colonies are entire, glistening, circular, and smooth. The requirement for tryptophan has been checked.
Deposited as
Escherichia coli (Migula) Castellani and ChalmersDepositorsNCIMBChain of custodyATCC <-- NCIMB <-- M.H.L. Green <-- E.M. Witkin