Genomic DNA from Bacteroides thetaiotamicron strain VPI 5482 拉丁名
29148D-5 编号
Product category
Bacteria
Product type
Nucleic acid
Derived from
Bacteroides thetaiotaomicron VPI 5482 [CIP 104206T, E50, NCTC 10582] (ATCC 29148)
Organism
Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers
Type strain 模式菌株 Yes
Genome sequenced strain
Yes
Applications
Bioinformatics
Next-generation sequencing
Mass 规格 5 μg
Product format 提供形式 Dried
Shipping information
Stored in 1X TE buffer
Comments
This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction
(PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
Genome sequenced strainHandling procedure
Centrifuge tube prior to opening to prevent loss of pelleted material
Rehydrate contents of vial with molecular grade H2O.
Place vial at 37°C for 1 hour or at 2°C to 8°C overnight.
For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.
Resuspending the dried DNA in ≥250 µL may give better results.
Handling notes
Genomic DNA isolated from bacteria is appropriate for PCR* and other molecular biology applications.
*The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche Inc.
Use of the PCR process requires a license.
Depositor of Source Strain: EP CatoTotal amount
Total DNA by PicoGreen® measurement was found to be approximately 5 µg.
Purity (A260/A280)
1.6 to 2.0
Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome SequencingDeposited as
Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers
Depositors
ATCC
Cross references
GenBank AE015928
Bacteroides thetaiotaomicron VPI-5482, complete genome.