ATCC® Number:VR-1355™
Classification: Chlamydiacea, Chlamydophila
Agent: Chlamydophila pneumoniae deposited as Chlamydia pneumoniae Grayston et al.
Strain: TWAR strain 2043
Original Source: nasopharynx of human patient with pneumonia
Depositors: MR Hammerschlag
Biosafety Level:2
Shipped: frozen
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Host Organism : HEp-2 cells (ATCCCCL-23) HeLa 229 cells (ATCCCCL-2.1) human; human cell lines
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 35.0°C Duration: 3 days
Effect : Yes, in vitro effects: Cytopathic effects (intracellular inclusions) in tissue culture which may be visualized by appropriate conjugated antibodies
Comments : Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS (genus antigen: Kallestad C. trachomatis Culture Confirmation System). Will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1-2ug/ml) in medium. This preparation has tested positive for Mycoplasma contamination.
References : 32842: Chirgwin K, et al. In vitro susceptibilities of Chlamydia pneumoniae (Chlamydia sp. strain TWAR). Antimicrob. Agents Chemother. 33: 1634-1635, 1989. PubMed: 2817862