ATCC? Number:VR-1361?
Classification: Thiotrichales, Piscirickettsiaceae, Piscirickettsia
Agent: Piscirickettsia salmonis Fryer et al.
Strain: LF-89
Original Source: clinical specimen - animal (Isolated by J. L. Fryer and C. N. Lannan from the kidney of a Coho salmon (Oncorhynchus kisutch) displaying lethargy, anemia, swollen kidney, splenomegaly, darkened coloration and rare hepatic lesions.)Chile Isolation date: October, 1989
Depositors: JL Fryer, CN Lannan
Biosafety Level:2 [This is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist. ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Atmosphere: air, 95%; carbon dioxide (CO2), 5% Max Temperature: 18.0°C Min Temperature: 15.0°C Duration: 6 to 14 days; For best results host cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture (small refractile rounding and clumping)
Comments : This organism is a gram-negative, non-motile, pleomorphic 0.5 to 1.5 micometer diameter coccoid.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020
References : 29882: Fryer JL, Mauel MJ. The rickettsia: an emerging group of pathogens in fish. Emerg. Infect. Dis. 3: 137-144, 1997. PubMed: 9204294 32850: Fryer JL, et al. Isolation of a rickettsiales-like organism from diseased Coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathol. 25: 107-114, 1990. 91898: Fryer JL, et al. Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. Int. J. Syst. Bacteriol. 42: 120-126, 1992. PubMed: 1371057