ATCC® Number:VR-903™
Classification: Chlamydiaceae
Agent: Chlamydia trachomatis (Busacca) Rake deposited as Lymphogranuloma venereum type III
Strain: Lymphogranuloma venereum (LGV III) strain 404
Original Source: Lymph node from human with LGV
Depositors: J Schachter
Biosafety Level:2
Shipped: frozen
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Host Organism : Mus musculus
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-3 days; For best results in tissue culture, cells should be 24-48 hours old and 80-100% confluent. The cells will not continue to grow after adding cycloheximide.
Effect : Yes, in vitro effects: Cytopathic effects, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen round middle. Inclusions best visualized by fluorescent antibody.
Store at : -70°C (or colder)
References : 33998: Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618 33999: Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529