ATCC® Number:VR-1544™
Classification: Herpesvirus, Human
Agent: Herpes simplex virus 1
Strain: GHSV-UL46
Depositors: M Willard
Biosafety Level:2
Shipped: frozen
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Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-4 days; For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Effect : Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing)
Store at : -70°C (or colder)
Comments : Incubation is approximately four days when monolayers are infected at multiplicity of infection (MOI) of 0.01 This virus makes green fluorescent plaques. The virus produced is labeled with GFP in the tegument protein VP11/12.
References : 61301: Willard M. Rapid directional translocations in virus replication.. J. Virol. 76: 5220-5232, 2002. PubMed: 11967336