ATCC® Number:VR-1360™
Classification: Chlamydiacea, Chlamydophila
Agent: Chlamydophila pneumoniae deposited as Chlamydia pneumoniae Grayston et al.
Strain: CM-1
Original Source: sputum from 58-year-old male with pneumonia
Depositors: KH Wong
Biosafety Level:2
Shipped: frozen
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Host Organism : HeLa 229 cells (ATCCCCL-2.1); HEp-2 cells (ATCCCCL-23)
Incubation : Duration: 3 days at 35.0-37.0°C
Effect : Yes, in vitro effects: Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Store at : -70.0°C or lower
Comments : This isolate was identified as C. pneumoniae by culture characteristics, morphology of inclusion bodies and reaction with monoclonal antibodies developed in CDC labs. C. pneumoniae CM-1 was isolated at the CDC, Atlanta on cycloheximide-treated HeLa 229 cells, and found to be serologically identical to the reference strain TW183 by the micro-immunofluorescent test. -70.0°C or lower