Trichomonas vaginalis Donne
(ATCC? 30001?)
Strain Designations 别名 C-1:NIH
Application 用途 Produces glucokinase ; Produces ketohexokinase fructokinase ; Maltose utilization;
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation 分离基物 Vaginal exudate from human adult female with acute vaginitis, 1956
Product Format 提供形式 frozen
Storage Conditions 保藏条件 Frozen
Cultures 冷冻物: -70℃ for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures 冻干物: 2-8℃
Live Cultures: See Protocols section for handling information
Type Strain 模式菌株 no
Comments 注释 Phylogeny based upon superoxide dismutase gene sequence analysis ; Diagnosis of Trichomonas vaginalis by PCR methods ; Inorganic pyrophosphatase ; Hydrogenosomal succinate thiokinase ; Glucokinase and fructokinase ; Differences in strains virulence ; Maltose utilization ; Carbon metabolism based on carbon source ; Carbohydrate metabolism in chemostats ; Antioxidant defenses ; Metronidazole radical anion generation ; Primary structure of the pyruvate:ferredoxin oxidoreductase ; Primary structure of the hydrogenosomal malic enzyme ; Response of lymphocytes ; Fructose-2,6-bisphosphate-insensitive pyrophosphate:fructose-6-phosphate phosphotransferase A linear double-stranded RNA ; Viability at four temperatures ; Extranuclear DNA ; Effect of oxygen and carbon dioxide on growth ; Virus RNAs ; Properties of a secondary alcohol dehydrogenase ; Cytochemistry of hydrogenosomal enzymes ; Ferredoxin-dependent reduction of nitroimidazole derivatives ; Subcellular localization of enzymes of the arginine dihydrolase pathway ; In vitro susceptibility to metronidazole;
Medium 培养基 ATCC? Medium 2154: LYI Entamoeba medium
Growth Conditions 生长条件
Temperature 培养温度: 35℃
Atmosphere 需氧情况: Microaerophilic
Culture System: Axenic; pH 6 Cryopreservation Harvest and Preservation
1,Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.,
2,Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
3,While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.,
a,Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube;
b,Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium;
c,Invert several times to dissolve the DMSO;
d,Allow to warm to room temperature.
4,Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.,
5,Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).,
6,Place the vials in a controlled rate freezing unit. From room temperature cool at -1℃/min to -40℃. If the freezing unit can compensate for the heat of fusion, maintain rate at -1℃/min through the heat of fusion. At -40℃ plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1℃ freezing apparatus. Place the apparatus at -80℃ for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1℃/min.)
7,The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130℃ are stabile indefinitely. Those stored at temperatures above -130℃ are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55℃.
8,To establish a culture from the frozen state place an ampule in a water bath set at 35℃. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.,
9,Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.
,10,Incubate the culture at 35?C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15? horizontal slant for medium 2154).
Name of Depositor 寄存人 LS Diamond
Special Collection NCRR Contract
Chain of Custody ATCC <-- LS Diamond <-- TA Burch/L.V. Reardon <-- L Jacobs
Year of Origin 1956
References Searle SM, Muller M. Inorganic pyrophosphatase of Trichomonas vaginalis. Mol. Biochem. Parasitol. 44: 91-96, 1991. PubMed: 1849232
Lloyd D, Pedersen JZ. Metronidazole radical anion generation in vivo in Trichomonas vaginalis: oxygen quenching is enhanced in a drug-resistant strain. J. Gen. Microbiol. 131: 87-92, 1985. PubMed: 2985740
Jenkins TM, et al. Hydrogenosomal succinate thiokinase in Tritrichomonas foetus and Trichomonas vaginalis. Biochem. Biophys. Res. Commun. 179: 892-896, 1991. PubMed: 1898409
Mertens E, Muller M. Glucokinase and fructokinase of Trichomonas vaginalis and Tritrichomonas foetus. J. Protozool. 37: 384-388, 1990. PubMed: 2213652
Reardon LV, et al. Differences in strains of Trichomonas vaginalis as revealed by intraperitoneal injections into mice. J. Parasitol. 47: 527-532, 1961. PubMed: 13740097
Hrdy I, Muller M. Primary structure and eubacterial relationships of the pyruvate:ferredoxin oxidoreductase of the amitochondriate eukaryote Trichomonas vaginalis. J. Mol. Evol. 41: 388-396, 1995. PubMed: 7563125
ter Kuile BH, Muller M. Maltose utilization by extracellular hydrolysis followed by glucose transport in Trichomonas vaginalis. Parasitology 110: 37-44, 1995. PubMed: 7845710
ter Kuile BH. Carbohydrate metabolism and physiology of the parasitic protist Trichomonas vaginalis studied in chemostats. Microbiology 140: 2495-2502, 1994. PubMed: 7952199
ter Kuile BH. Adaptation of the carbon metabolism of Trichomonas vaginalis to the nature and availability of the carbon source. Microbiology 140: 2503-2510, 1994. PubMed: 7952200
Ellis JE, et al. Antioxidant defences in the microaerophilic protozoan Trichomonas vaginalis: comparison of metronidazole-resistant and sensitive strains. Microbiology 140: 2489-2494, 1994. PubMed: 7952198
Yarlett N, et al. Metronidazole-resistant clinical isolates of Trichomonas vaginalis have lowered oxygen affinities. Mol. Biochem. Parasitol. 19: 111-116, 1986. PubMed: 3487729
Hrdy I, Muller M. Primary structure of the hydrogenosomal malic enzyme of Trichomonas vaginalis and its relationship to homologous enzymes. J. Eukaryot. Microbiol. 42: 593-603, 1995. PubMed: 7581334
Muller M, Lindmark DG. Uptake of metronidazole and its effect on viability in Trichomonads and Entamoeba invadens under anaerobic and aerobic conditions. Antimicrob. Agents Chemother. 9: 696-700, 1976. PubMed: 1083712
Yarlett N, et al. Subcellular localization of the enzymes of the arginine dihydrolase pathway in Trichomonas vaginalis and Trtrichomonas foetus. J. Eukaryot. Microbiol. 41: 554-559, 1994. PubMed: 7866382
Yano A, et al. Antigen-specific proliferation responses of peripheral blood lymphocytes to Trichomonas vaginalis antigen in patients with Trichomonas vaginalis. J. Clin. Microbiol. 17: 175-180, 1983.PubMed: 6601112
Lloyd D, et al. Metronidazole-resistant clinical isolates of Trichomonas vaginalis maintain low intracellular metronidazole radical anion levels as a consequence of defective oxygen scavenging. Biologica 30: 521-528, 1988.
Mertens E, et al. Presence of a fructose-2,6-bisphosphate-insensitive pyrophosphate: fructose-6-phosphate phosphotransferase in the anaerobic protozoa Tritrichomonas foetus, Trichomonas vaginalis and Isotricha prostoma. Mol. Biochem. Parasitol. 37: 183-190, 1989. PubMed: 2558319
Wang AL, Wang CC. A linear double-stranded RNA in Trichomonas vaginalis. J. Biol. Chem. 260: 3697-3702, 1985. PubMed: 2982874
Smith RF. Viability of Trichomonas vaginalis in vitro at four temperatures. J. Clin. Microbiol. 18: 834-836, 1983. PubMed: 6605364
Turner G, Muller M. Failure to detect extranuclear DNA in Trichomonas vaginalis and Tritrichomonas foetus. J. Parasitol. 69: 234-236, 1983. PubMed: 6600788
Mack SR, Muller M. Effect of oxygen and carbon dioxide on the growth of Trichomonas vaginalis and Tritrichomonas foetus. J. Parasitol. 64: 927-929, 1978. PubMed: 309937
Gelbart SM, et al. Growth of Trichomonas vaginalis in commercial culture media. J. Clin. Microbiol. 28: 962-964, 1990. PubMed: 2351739
Tai JH, et al. The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis. Exp. Parasitol. 76: 278-286, 1993. PubMed: 8500587
Kleiner DE, Johnston M. Purification and properties of a secondary alcohol dehydrogenase from the parasitic protozoan Tritrichomonas foetus. J. Biol. Chem. 260: 8038-8043, 1985. PubMed: 3159722
Goosen NK, et al. Effect of fixation on activity and cytochemistry of hydrogenosomal enzymes in Trichomonas vaginalis. J. Gen. Microbiol. 136: 2189-2193, 1990. PubMed: 1706759
Yarlett N, et al. Ferredoxin-dependent reduction of nitroimidazole derivatives in drug-resistant and susceptible strains of Trichomonas vaginalis. Biochem. Pharmacol. 35: 1703-1708, 1986. PubMed: 3486660
Viscogliosi E, et al. Phylogenetic implication of iron-containing superoxide dismutase genes from trichomonad species. Mol. Biochem. Parasitol. 80: 209-214, 1996. PubMed: 8892298
Roger AJ, et al. A possible mitochondrial gene in the early-branching amitochondriate protist Trichomonas vaginalis. Proc. Natl. Acad. Sci. USA 93: 14618-14622, 1996. PubMed: 8962102
Lloyd D, Kristensen B. Metronidazole ingibition of hydrogen production in vivo in drug-sensitive and resistant strains of Trichomonas vaginalis. J. Gen. Microbiol. 131: 849-853, 1985.
Vanacova S, et al. Characterization of Trichomonad species and strains by PCR fingerprinting. J. Eukaryot. Microbiol. 44: 545-552, 1997. PubMed: 9435127
Harmych SE, et al. Lactate dehydrogenase from the protozoan parasite, Trichomonas vaginalis. Comp. Biochem. Physiol. 115B: 405-409, 1996.
ter Kuile BH. Metabolic adaptation of Trichomonas vaginalis to growth rate and glucose availability. Microbiology 142: 3337-3345, 1996. PubMed: 9004498 T
er Kuile BH, Muller M. Interaction between facilitated diffusion of glucose across the plasma membrane and its metabolism in Trichomonas vaginalis. FEMS Microbiol. Lett. 110: 27-32, 1993. PubMed: 8319891
Madico G, et al. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J. Clin. Microbiol. 36: 3205-3210, 1998. PubMed: 9774566
van Leeuwen F, et al. beta-D-glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres. Proc. Natl. Acad. Sci. USA 95: 2366-2371, 1998. PubMed: 9482891
Keeling PJ, et al. Linked genes for calmodulin and E2 ubiquitin-conjugating enzyme in Trichomonas vaginalis. J. Eukaryot. Microbiol. 43: 468-474, 1996. PubMed: 8976604
Marinets A, et al. The sequence and organization of the core histone H3 and H4 genes in the early branching amitochondriate protist Trichomonas vaginalis. J. Mol. Evol. 43: 563-571, 1996. PubMed: 8995053
Keeling PJ, et al. Evolutionary relationship between translation initiation factor eIF-2gamma and selenocysteine-specific elongation factor SELB: change of function in translation factors. J. Mol. Evol. 47: 649-655, 1998. PubMed: 9847405
Edgell DR, et al. Evidence of independent gene duplications during the evolution of archael and eukaryotic family B DNA polymerases. Mol. Biol. Evol. 15: 1207-1217, 1998. PubMed: 9729885
Mertens E, et al. The pyrophosphate-dependent phosphofructokinase of the protist, Trichomonas vaginalis, and the evolutionary relationships of protist phosphofructokinases. J. Mol. Evol. 47: 739-750, 1998. PubMed: 9847416
Wu G, et al. Convergent evolution of Trichomonas vaginalis lactate dehydrogenase from malate dehydrogenase. Proc. Natl. Acad. Sci. USA 96: 6285-6290, 1999. PubMed: 10339579
Viscogliosi E, Mueller M. Phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. J. Mol. Evol. 47: 190-199, 1998. PubMed: 9694668
Archibald JM, et al. Origin and evolution of eukaryotic chaperonins: phylogenetic evidence for ancient duplications in CCT genes. Mol. Biol. Evol. 17: 1456-1466, 2000. PubMed: 11018153
Bouma MJ, et al. Activity of disulfiram (bis(diethylthiocarbamoyl)disulphide) and ditiocarb (diethyldithiocarbamate) against metronidazole-sensitive and -resistant Trichomonas vaginalis and Tritrichomonas foetus. J. Antimicrob. Chemother. 42: 817-820, 1998. PubMed: 10052908
Tachezy J, et al. Cattle pathogen Tritrichomonas foetus (Riedmuller, 1928) and pig commensal Tritrichomonas suis (Gruby & Delafond, 1843) belong to the same species. J. Eukaryot. Microbiol. 49: 154-163, 2002. PubMed: 12046599
Sanchez LB, et al. Fructose-1,6-bisphosphate aldolases in amitochondriate protists constitute a single protein subfamily with eubacterial relationships. Gene 295: 51-59, 2002. PubMed: 12242011
Gerbod D, et al. Phylogenetic relationships of class II fumarase genes from trichomonad species. Mol. Biol. Evol. 18: 1574-1584, 2001. PubMed: 11470849
Cornelius DC, et al. Short report: genetic relatedness of Trichomonas vaginalis reference and clinical isolates. Am. J. Trop. Med. Hyg. 83: 1283-1286, 2010.
Cross References Nucleotide (GenBank) : Z70670 T.vaginalis sod1 gene.
Nucleotide (GenBank) : Z70671 T.vaginalis sod2 gene.
Nucleotide (GenBank) : Z70672 T.vaginalis sod3 gene.
Nucleotide (GenBank) : Z70673 T.vaginalis sod4 gene.
Nucleotide (GenBank) : Z70674 T.vaginalis sod5 gene.
Nucleotide (GenBank) : M33717 T.vaginalis ferredoxin gene, complete cds.
Nucleotide (GenBank) : U57000 chaperonin 60 (cpn60) mRNA, partial coding sequence
Nucleotide (GenBank) : AF067404 DNA polymerase epsilon gene, partial coding sequence
Nucleotide (GenBank) : X98016 histone H4-2 and histone H3-2, partial coding sequence
Nucleotide (GenBank) : U27577 Trichomonas vaginalis polyubiquitin (UbA) mRNA, partial cds.
Nucleotide (GenBank) : U28008 Trichomonas vaginalis ubiquitin 1A (Ub1A) gene, partial cds.
Nucleotide (GenBank) : U28009 Trichomonas vaginalis ubiquitin 1C (Ub1C) gene, partial cds.
Nucleotide (GenBank) : U28010 Trichomonas vaginalis ubiquitin 1D (Ub1D) gene, partial cds.
Nucleotide (GenBank) : U28011 Trichomonas vaginalis ubiquitin 1E (Ub1E) gene, partial cds.
Nucleotide (GenBank) : U28012 Trichomonas vaginalis ubiquitin dimer 2B (Ub2B) gene, partial cds. Nucleotide (GenBank) : U70308 Trichomonas vaginalis mitochondrial-type HSP70 mRNA, complete cds.
Nucleotide (GenBank) : U38692 Trichomonas vaginalis cytosolic malate dehydrogenase gene, complete cds.
Nucleotide (GenBank) : U28013 Trichomonas vaginalis polyubiquitin junction JC (UbJC) gene, partial cds.
Nucleotide (GenBank) : AF060233 Trichomonas vaginalis L-lactate dehydrogenase (LDH1) gene, complete cds.
Nucleotide (GenBank) : AF058282 Trichomonas vaginalis elongation factor 1 alpha (tef1) mRNA, partial cds.
Nucleotide (GenBank) : L11394 Trichomonas vaginalis glyceraldehyde 3-phosphate dehydrogenase mRNA, 3' end.
Nucleotide (GenBank) : U07784 Trichomonas vaginalis ATCC 30001 ferredoxin (FD) gene, Inr promoter element.
Nucleotide (GenBank) : M97553 Trichomonas vaginalis succinyl-CoA synthetase beta-subunit gene, complete cds.
Nucleotide (GenBank) : U07785 Trichomonas vaginalis ATCC 30001 P-glycoprotein (Pgp1) gene, promoter element.
Nucleotide (GenBank) : AF022421 glyceraldehyde-3-phosphate dehydrogenase (gap3) gene, partial coding sequence
Nucleotide (GenBank) : U07782 Trichomonas vaginalis ATCC 30001 beta-tubulin (beta-Tub) gene, promoter element.
Nucleotide (GenBank) : U07780 Trichomonas vaginalis ATCC 30001 alpha-tubulin (alpha-Tub) gene, promoter element.
Nucleotide (GenBank) : U07203 Trichomonas vaginalis hydrogenosomal adenylate kinase proprotein gene, complete cds.
Nucleotide (GenBank) : U16836 Trichomonas vaginalis hydrogenosomal malic enzyme subunit A (maeA) gene, complete cds.
Nucleotide (GenBank) : AF022414 Trichomonas vaginalis glyceraldehyde-3-phosphate dehydrogenase (gap2) gene, partial cds.
Nucleotide (GenBank) : AF005075 translation initiation factor 2 gamma subunit (eIF-2 gamma) gene, partial coding sequence
Nucleotide (GenBank) : U38786 calmodulin (CAM) and E2 ubiquitin-conjugating enzyme (TvUBC) genes, partial coding sequence
Nucleotide (GenBank) : AF053370 PPi-dependent fructose 6-phosphate 1-phosphotransferase (pfk3) gene, partial coding sequence
Nucleotide (GenBank) : U16822 Trichomonas vaginalis pyruvate:ferredoxin oxidoreductase proprotein (pfoA) gene, complete cds.
Nucleotide (GenBank) : U16823 Trichomonas vaginalis pyruvate:ferredoxin oxidoreductase proprotein (pfoB) gene, complete cds.
Nucleotide (GenBank) : U16838 Trichomonas vaginalis hydrogenosomal malic enzyme subunit C proprotein (maeC) gene, partial cds.
Nucleotide (GenBank) : U16839 Trichomonas vaginalis hydrogenosomal malic enzyme subunit D proprotein (maeD)gene, partial cds.
Nucleotide (GenBank) : U16837 Trichomonas vaginalis hydrogenosomal malic enzyme subunit B proprotein (maeB) gene, complete cds.
Nucleotide (GenBank) : U07783 Trichomonas vaginalis ATCC 30001 70kDa cystolic heat shock protein (cHSP70 gene), promoter element.
Nucleotide (GenBank) : AF044973 pyrophosphate-dependent fructose 6-phosphate 1-phosphotransferase (Pfk1) gene, complete coding sequence
Nucleotide (GenBank) : AF053371 Trichomonas vaginalis PPi-dependent fructose 6-phosphate 1-phosphotransferase (pfk2) gene, partial cds.
Nucleotide (GenBank) : U07781 Trichomonas vaginalis ATCC 30001 succinyl CoA synthetase beta subunit (beta-SCS) gene, Inr promoter element.
Nucleotide (GenBank) : U07779 Trichomonas vaginalis ATCC 30001 succinyl CoA synthetase alpha subunit (alpha-SCSB) gene, Inr promoter element.
固定电话:010-53515223
业务手机:18610886853,18610241664,18701099593,18971386853,18701098095
微信号码:vrmte521(18610886853企业微信);18610241664;biobw0905(18701098095)
企业公众号:800185668
官方邮箱:biobw11@sina.com(微生物菌种查询网)
投诉电话:biobw8888(18701099593)