pOT2
(ATCC® PVMC-1™)
Organism: Odontoglossum ringspot virus /
Clone Type: Clone /
Depositors: SM Wong
Designations pOT2 Species Odontoglossum ringspot virus
Depositors SM Wong
Applications contains sequence
Plant research
Vector Construct size (kb): 6.0
Plasmid const
ructed by inserting full-length cDNA clone of ORSV into pBlueScript plasmid
Insert DNA: cDNA
Insert lengths(kb): 3.0
Tissue: Cattleya odontoglossum ringspot Disease: odontoglossum ringspo
Gene product: [ORSV clone] Insert Size (kb) 3.0
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments Highly infectious in vitro RNA transcripts can be obtained using Promega's In Vitro Transcription kit (T7 RNA polymerase). Plasmid constructed by inserting full-length cDNA clone of odontoglossum ringspot tobamovirus (ORSV) into pBluescript KS+ vector. Classification Tobamovirus
References Yu HH, Wong SM. A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene. Arch. Virol. 143: 163-171, 1998. PubMed: 9505974