Rhizopus stolonifer 菌株拉丁名 (Ehrenberg : Fries) Lind
(ATCC 6227b 菌株编号
Permits and Restrictions View Permits
Deposited As Rhizopus nigricans Ehrenberg, teleomorph
Strain Designations 菌株别名 C-25B [CBS 382.52, IFO 5781, IFO 6154, IFO 6300, IMI 57762, VKM F-401]
Application produces 11-alpha-hydroxylase produces 17 beta-hydroxysteroid dehydrogenase
Estradiol 17-beta-dehydrogenase-1, Hydroxysteroid dehydrogenase 17 beta, type 1, hydroxysteroid 17-beta dehydrogenase 1 produces chiral alcohols produces steroids transformation of sesquiterpene lactone costunolide reduces 3-methoxy-8(14)-seco-1,3,5(10),9(11)-estratetraene-14,17-dione
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format提供形式 freeze-dried
Type Strain模式菌株 no
Preceptrol? no
Mating Type mating type plus
Comments 注释 Hydroxylation of 17 alpha-ethynyl steroids Detoxification of steroids by cytochrome P45011alpha
Medium 培养基 ATCC Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生长条件
Temperature 培养温度: 24.0℃
Name of Depositor AF Blakeslee Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims.
Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Cross References参考文献 Nucleotide (GenBank) : Y10414 R.nigricans mRNA for stress protein.
Nucleotide (GenBank) : S50228 steroid hydroxylase cytochrome P-450 [Rhizopus nigricans, ATCC References Abul-Hajj YJ. Stereochemistry of C-1, 2 dehydrogenation of 5 beta-pregnane-3, 11, 20-trione by Septomyxa affinis. J. Biol. Chem. 247: 686-691, 1972. PubMed: 5058221
Zakelj-Mavric M, et al. The bioconversion of 17 alpha-ethynyl steroids with 11 alpha-hydroxylase of Rhizopus nigricans. FEMS Microbiol. Lett. 33: 117-120, 1986.
Mills JS. 10-Fluoro-19-nor steroids. US Patent 3,053,858 dated Sep 11 1962
Gibian H, et al. Production of optically active antipodes. US Patent 3,562,112 dated Feb 9 1971
Lanisnik T, et al. Fungal 17 beta-hydroxysteroid dehydrogenase. FEMS Microbiol. Lett. 78: 49-52, 1992. PubMed: 1334892
Breskvar K, et al. The role of cytochrome P450(11 alpha) in detoxification of steroids in the filamentous fungus Rhizopus nigricans. J. Steroid Biochem. Mol. Biol. 52: 271-275, 1995. PubMed: 7696148
Hudnik-Plevnik T, Cresnar B. Factors affecting the induction of 11 alpha-hydroxylase of progesterone in the filamentous fungus Rhizopus nigricans. J. Steroid Biochem. 35: 749-751, 1990. PubMed: 2362437
Clark AM, Hufford CD. Microbial transformations of the sesquiterpene lactone costunolide. J. Chem. Soc. Perkin Trans. 1979: 3022-3028, 1979.
Kasai M, et al. Enantioselective ester hydrolyses using Rhizopus nigricans: stereoselective synthesis and absolute stereochemistry of (-)-cis-and (-)-trans-1-hydroxy-4-methyl-1,2,3,4-tetrahydronaphthalene. Can. J. Chem. 63: 1287-1291, 1985.
Zakelj-Mavric M, Belic I. Hydroxylation of steroids with 11alpha-hydroxylase of Rhizopus nigricans. J. Steroid Biochem. 28: 197-201, 1987. PubMed: 3498094