Clostridium difficile (Hall and O'Toole) Prevot 拉丁名
(ATCC? 700057?) 编号
Strain Designations 菌株别名 VPI 11186 [6]
Application 用途 Susceptibility testing
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冻干物:2°C to 8°C
Live Culture 活菌 :See Propagation Section
Preceptrol? no
Type Strain 模式菌株 no
Comments 注释 Toxinotype tcdA-, tcdB-
Ribotype 038
Binary toxin gene cdtB not amplified by PCR
Nontoxigenic
Medium 培养基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions 生长条件
Temperature 培养温度: 37°C
Atmosphere 需氧情况 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor 寄存人 LV Moore
Special Collection NCRR Contract
Chain of Custody 来源国家 ATCC <-- LV Moore <-- H Beerens 6
References 参考文献 Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
BD GeneOhm Cdiff. BD Becton Dickinson.
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile
Ribotyping Network for England and N. Ireland (CDRN). An
enhanced DNA fingerprinting service to investigate potential
Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986