细胞名称:AB2.2小鼠胚胎干细胞AB2.2
描述;小鼠全能性胚胎干细胞,每支细胞量1×106
动物种别;小鼠,129S5/SvEvBrd(原129/SvEvBrd)品系
性别:雄
组织来源:小鼠胚胎内细胞团
形态:球形克隆
培养基和添加剂:mES完全培养液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
供应限制 A。仅供研究之用。
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中文名称:小鼠胚胎干细胞AB2.2
提供形式:复苏状态