生长状态:悬浮生长
品系:BALB/c
细胞类型:B淋巴细胞
是否是肿瘤细胞:0
物种来源:小鼠
细胞形态:淋巴样
ATCC Number:TIB-50?
相关疾病:淋巴瘤
数量:大量
运输方式:冻存运输
器官来源:脾
规格:0.5ml Designations: RAW 8.1
Depositors: ?P Ralph
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Organ: spleen
Strain: BALB/c
Disease: lymphoma
Cell Type: B lymphocyte;
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Comments:RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus.
Growth of this line is inhibited by 5 mcg/ml LPS.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1137: Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429
2104: Ralph P, et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. PubMed: 4616699