数量:大量
年限:54 years
ATCC Number:CCL-199?
运输方式:冻存运输
相关疾病:上皮细胞癌
是否是肿瘤细胞:1
物种来源:人
器官来源:肺
生长状态:贴壁生长
细胞形态:成纤维样
规格:0.1ml Designations: HLF-a
Depositors: ?GD Stoner
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: epidermoid carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: February, 1976
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12,13
D5S818: 8,12
D7S820: 9,12
THO1: 6,9.3
TPOX: 6,8
vWA: 15
Cytogenetic Analysis:human female; diploid; stable
Isoenzymes: G6PD, B
Age: 54 years
Gender: female
Ethnicity: Black
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020