运输方式：冻存运输
数量：大量
年限：13 years
ATCC Number：CRL-1544?
相关疾病：骨肉瘤
生长状态：贴壁生长
器官来源：骨
是否是肿瘤细胞：1
物种来源：人
规格：10 μg Designations: KHOS/NP (R-970-5)
Depositors: ?JS Rhim
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments:This cell line was derived from HOS (ATCC CRL-1543 ) by transformation using Kirsten murine sarcoma virus (Ki-MSV).
The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice.
The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses.
Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 22366: Rhim JS, et al. Non-producer human cells induced by murine sarcoma virus. Int. J. Cancer 15: 23-29, 1975. PubMed: 165148
22851: Rhim JS, et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. PubMed: 171229
