年限:13 year old
运输方式:冻存运输
器官来源:骨
ATCC Number:CRL-8304?
相关疾病:骨肉瘤
是否是肿瘤细胞:1
物种来源:人
细胞形态:成纤维样
数量:大量
生长状态:贴壁生长
规格:10 μg Designations: 143B PML BK TK
Depositors: ?Wistar Institute
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Age: 13 year old
Gender: female
Ethnicity: Caucasian
Comments:Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products:plasmid in bacteria:ATCC 39369
parental cell line:ATCC CRL-8303