KHOS/NP(人骨肉瘤细胞)
产品类别:人源细胞系
生长特性:贴壁生长
细胞形态:纤维
冻存条件:90%FBS+10%DMSO
Synonyms:KHOS/NP(人骨肉瘤细胞)
Background:This cell line was derived from HOS (ATCC CRL-1543) by transformation using Kirsten murine sarcoma virus (Ki-MSV).
Species:Homo sapiens, human
Tissue:bone
Disease:osteosarcoma
Gender:female, 13 years, Caucasian
Growth Mode:adherent
DNA Profile:Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
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Culture Medium:MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Tumor Formation:Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments:The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice.
Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens.