LB,37℃,卡那
启动子:ADH1
复制子:2U
质粒分类:酵母系列,酵母双杂交载体
质粒大小:7.9kb
原核抗性:Kan
筛选标记:TRP1
克隆菌株:DH5a
培养条件:37度
表达宿主:酵母细胞
5'测序引物:T7:TAATACGACTCACTATAGGG
3'测序引物:根据序列设计引物
质粒属性
载体宿主:酵母菌
载体用途:杂交
基因种属:
基因类型:
原核抗性:Kan
真核抗性:TRP1
荧光蛋白:
质粒简介
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.