LB,37℃,氨苄
别称:
酵母单杂交
启动子:
TRP1 ,T7,T3
复制子:
pUC
质粒分类:
酵母系列,酵母单杂交载体
质粒大小:
7207bp
原核抗性:
Kan
筛选标记:
TRP1
克隆菌株:
DH5a
培养条件:
37度
表达宿主:
酵母细胞
5'测序引物:
M13F:TGTAAAACGACGGCCAGT
3'测序引物:
根据序列设计引物
质粒属性
载体宿主:
酵母菌
载体用途:
杂交
基因种属:
基因类型:
原核抗性:
Kan
真核抗性:
TRP1
荧光蛋白:
质粒简介
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.