Catalog Number AS 107 LT
Intended Use
The EnviroLogix QuickStix Kit for Soybean Rust screens for the presence of
soybean rust caused by Phakopsora pachyrhizi. The strip test can detect the presence
of the pathogen at the very early stages of infection, from chlorotic lesions (before
formation of a pustule) to immature pustules (not releasing spores). During this
period it is often difficult yet critical to differentiate the soybean rust symptoms from
other diseases caused by bacterial, viral or fungal infections and/or insect damage. In
addition, the test can also be used to detect advanced rust symptoms with
uredinospores and teliospores, complementing visual inspections.
In controlled inoculation studies with levels as low as 100,000 spores/mL, this kit has
been shown to detect the presence of soybean rust infection before the appearance of
visual symptoms. Infection levels in the field may vary depending on environmental
conditions.
How the Test Works
This test is for use on leaf tissue that is suspected to be infected with soybean rust
spores. To detect infected tissue with the EnviroLogix QuickStix Strip, leaf tissue
must be extracted with the buffer provided.
Each QuickStix Strip has an absorbent pad at both ends. The protective tape with the
arrow indicates which end of the strip to insert into the extraction tube. The sample
travels up the membrane strip and is absorbed into the larger pad at the top of the
strip. The portion of the strip between the protective tape and the absorbent pad at
the top of the strip is used to view the reactions as described under “Interpreting the
Results”.
Sample Preparation
1. Select a leaf or portion of leaf that measures approximately 1” (2.5 cm) diameter
and place in mesh bag (use the end of the canister as a guide). Using a hard
object (e.g. coin) rub across the surface of the mesh bag against a hard surface
until the leaf has transparent areas where the mesh has been forced through.
2. Add 5 mL of Extraction Buffer directly into the mesh bag (this can be done by
adding 1 full draw with the provided transfer pipette). Massage the exterior of
the mesh bag with fingers while holding the top of the bag closed to prevent the
buffer from spilling.
3. Using the same pipette, fill the reaction vial with extract. Discard pipette and
mesh bag after use.
How to Run the QuickStix Strip Test
1. Allow refrigerated canisters and Extraction Buffer to come to room temperature
before opening. Remove the QuickStix Strips to be used. Avoid bending the
strips. Reseal the canister immediately.
2. Place the strip into the reaction vial. The sample will travel up the strip. Use the
cardboard rack provided to support multiple vials.
3. Allow the strip to develop for 10 minutes before making final assay
interpretations. Positive sample results may become obvious more quickly.
4. To retain the strip, cut off and discard the bottom section of the strip covered by
the arrow tape.
QuickStix Kit for Soybean Rust
Page 2 of 4
Rev. 08-09-07
Transfer liquid into vial
with pipette
Add a test strip to the vial,
wait 10 minutes
Any pink test line is
considered positive
Interpreting the Results
Development of the Control Line within 10 minutes
indicates that the strip has functioned properly. Any strip
that does not develop a Control Line should be discarded and
the sample re-tested using another strip.
If the sample extract contains Soybean Rust infection, a
second line (Test Line) will develop on the membrane strip
between the Control Line and the protective arrow tape. The
results should be interpreted as positive for soybean rust.
The Test Line will typically be lighter than the Control Line.
If no Test Line is observed after 10 minutes, the results
should be interpreted as negative for the sample tested. See
Precautions and Notes.
Kit Storage
This QuickStix Kit can be stored at room temperature, or refrigerated for a longer
shelf life. Note the shelf life on the kit box for each storage temperature. The kit
may be used in field applications; however, prolonged exposure to high temperatures
may adversely affect the test results. Do not open the desiccated canister until ready
to use the test strips.
Cross Reactivity
The kit does not cross react with Phakopsora meibomiae, or several other rust
infections caused by Uromyces, Puccinia and Melampsora species. No cross
reactivity was observed with other common fungal genera including Aspergillus,
Cercospora kikuchii, C. sojina, Fusarium, Penicillium, Peronospora mansurica,
Pseudomonas savastanoi pv. Glycinea, Rhizoctonia, Rhizopus, Septoria, and
Xanthomonas campestris pv. Glycinea. No cross reactivity has been observed with
similar-looking diseases such as frogeye leafspot, powdery mildew, downy mildew,
brown spot, bacterial blight, bacterial pustule.
Precautions and Notes
• This kit is designed for screening the presence or absence of Soybean Rust and is
not meant to be quantitative. As with all tests, it is recommended that results be
confirmed by alternate method.
• Important Note: The kit will detect its target pathogen regardless of the
pathogen’s viability. It should not be used to determine the efficacy of treatment
efforts, because although the pathogen may be rendered non-viable, the protein is
still present and will cause a positive result.
• The assay has been optimized to be used with the protocol provided in the kit.
Deviation from this protocol may invalidate the results of the test.
• The test is recommended to be used on single leaf sample only. Compositing or
pooling of samples is not recommended and may result in false negative.
• A strong positive result may safely be interpreted in as little as 5 minutes after
sample addition. It is not safe, however, to conclude that a sample is negative
before a full 10 minutes has elapsed. A weakly positive sample may require the
full 10 minutes for a distinct Test Line to appear.
• A negative result does not preclude the presence of soybean rust infection in other
areas or at other times.
• Protect all components from hot or cold extremes of temperature when not in use.
Do not leave in direct sunlight or in vehicle. A small portable cooler is
recommended for field testing applications to protect the kit from extreme
temperatures.
Any pink
Test Line is
a positive
result.
QuickStix Kit for Soybean Rust
Page 3 of 4
Rev. 08-09-07
For Technical Support
Contact Us At:
EnviroLogix
500 Riverside Industrial
Parkway
Portland, ME 04103-1486
USA
Tel: (207) 797-0300
Toll Free: 866-408-4597
Fax: (207) 797-7533
e-mail:
info@envirologix.com
website:
www.envirologix.com
LIMITED WARRANTY
EnviroLogix Inc. (“EnviroLogix”) warrants the products sold hereunder (“the Products”)
against defects in materials and workmanship when used in accordance with the applicable
instructions for a period not to extend beyond a product’s printed expiration date. If the
Products do not conform to this Limited Warranty and the customer notifies EnviroLogix
in writing of such defects during the warranty period, including an offer by the customer to
return the Products to EnviroLogix for evaluation, EnviroLogix will repair or replace, at its
option, any product or part thereof that proves defective in materials or workmanship
within the warranty period.
ENVIROLOGIX MAKES NO OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES
OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. The
warranty provided herein and the data, specifications and descriptions of EnviroLogix
products appearing in EnviroLogix published catalogues and product literature are
EnviroLogix’ sole representations concerning the Products and warranty. No other
statements or representations, written or oral, by EnviroLogix’ employees, agents or
representatives, except written statements signed by a duly authorized officer of
EnviroLogix Inc., are authorized; they should not be relied upon by the customer and are
not a part of the contract of sale or of this warranty.
EnviroLogix does not warrant against damages or defects arising in shipping or handling,
or out of accident or improper or abnormal use of the Products; against defects in products
or components not manufactured by EnviroLogix, or against damages resulting from such
non-EnviroLogix made products or components. EnviroLogix passes on to customer the
warranty it received (if any) from the maker thereof of such non-EnviroLogix made
products or components. This warranty also does not apply to Products to which changes
or modifications have been made or attempted by persons other than pursuant to written
authorization by EnviroLogix.
THIS WARRANTY IS EXCLUSIVE. The sole and exclusive obligation of EnviroLogix
shall be to repair or replace the defective Products in the manner and for the period
provided above. EnviroLogix shall not have any other obligation with respect to the
Products or any part thereof, whether based on contract, tort, strict liability or otherwise.
Under no circumstances, whether based on this Limited Warranty or otherwise, shall
EnviroLogix be liable for incidental, special, or consequential damages.
This Limited Warranty states the entire obligation of EnviroLogix with respect to the
Products. If any part of this Limited Warranty is determined to be void or illegal, the
remainder shall remain in full force and effect.
License
EnviroLogix has developed this kit using proprietary reagents.
EnviroLogix, the EnviroLogix logo, and QuickStix are trademarks of EnviroLogix
Inc.
© EnviroLogix 2007
QuickStix Kit for Soybean Rust
Page 4 of 4
Rev. 08-09-07
Alternative Method
APPENDIX
PUNCH CAP METHOD
For an alternative method of testing a suspected spot on a leaf, disposable
tissue extractors are available as an accessory item (ACC #002) with the
following protocol:
1. Sandwich a section of leaf tissue between the cap and body of the Disposable
Tissue Extractor tube. Include the suspected spot within the circle. Snap
two circular leaf punches by closing the cap.
2. Push the leaf punches down into the tapered bottom of the tube with the
pestle and grind by rotating the pestle against the sides of the tube with
twisting motions. Write the sample identification on the tube with a
waterproof marker. Use the blue foam provided in the kit to support multiple
tubes.
3. Add 0.5 mL of buffer to the tissue sample (use a pipette or otherwise fill vial
to the 0.5 mL mark). Insert the pestle into the tube and grind again.
Continue this process for 20 to 30 seconds or until the leaf tissue is well
ground.
4. Continue on to the “How to Run the QuickStix Strip Test” section. Insert the
QuickStix Strip into the punch cap vial; no need to transfer extract to the
reaction vial.