基本信息
别称:Plasmid#79873;pAX01-dCas9
启动子:xylA
原核抗性:Amp
筛选标记:Ery
克隆菌株:DH5a
培养条件:37度
表达宿主:枯草杆菌
质粒属性
载体宿主:枯草杆菌
载体用途:基因敲除
基因种属:
基因类型:
原核抗性:Amp
真核抗性:
荧光蛋白:
质粒简介
pJMP1质粒是一个枯草杆菌基因编辑质粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Journal