菌种名称: 星座链球菌星座亚种
拉丁文名: Streptococcus constellatus subsp. constellatus
其他编号: ATCC 27823
培养基编号: 35,114
培养温度: 37℃,厌氧
分离源: 脓性胸膜炎患者
生理生化特征:
培养基编号: 35
名称: Blond Agar (血琼脂培养基)
可用于培养菌种:
备注: Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10% pH 7.2 [Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze
. [注]猪心和猪脑浸汁法:
(1)新鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;
(2)新鲜猪脑除去血管,取200克加蒸馏水至1000毫升;
(3)50℃保温1小时,然后煮开15分钟;
(4)冷却后用纱布过滤备用。
培养基编号: 114
名称: 脑心浸出液培养基Brain Heart Infusion (BHI)