Name:
Clostridium chromiireducens Inglett et al. 2011
DSM No.:
110167
Strain designation:
NM79_F5
Isolated from:
caecal/colon content; C57BL/6, APCmin/+ Msh2-/-, 9-week-old mouse
Country:
Canada
Toronto
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- W. Navarre, Toronto; NM79_F5
Cultivation conditions:
Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT)., 37°C
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- W. Navarre, Toronto; NM79_F5. Caecal/colon content; C57BL/6, APCmin/+ Msh2-/-, 9-week-old mouse. Canada, Toronto. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT)., incubation time: 2-3 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic)
Risk group:
1 (classification according to German TRBA)