ATCC medium: 854 Blue-green medium (ATCC medium 616) with vitamin B12
ATCC Medium 616 (see below) with 10 mg/L of vitamin B12. Add filter-sterilized vitamin B12 to cooled medium.
ATCC Medium 616: NaNO3 ..............................1.5 g
K2HPO4 .............................0.04 g
MgSO4 . 7H2O .......................0.075 g
CaCl2 . 2H2O .......................0.036 g
Citric acid........................6.0 mg
Ferric ammonium citrate............6.0 mg
EDTA...............................1.0 mg
Na2CO3 .............................0.02 g
Trace Metal Mix A5 (see below).....1.0 ml
Agar (if needed)..................10.0 g
Distilled water....................1.0 L
Adjust final pH to 7.1. Autoclave at 121C for 15 minutes.
Trace Metal Mix A5: H3BO3 ..............................2.86 g
MnCl2 . 4H2O .......................1.81 g
ZnSO4 . 7H2O .......................0.222 g
Na2MoO4 . 2H2O......................0.39 g
CuSO4 . 5H2O .......................0.079 g
Co(NO3)2 . 6H2O....................49.4 mg
Distilled water....................1.0 L