ATCC Medium: 1914 Revised BSK Medium
ATCC currently uses Sigma # B-8291 BSK-H Complete medium. This medium is purchased in 500ml volumes and stored frozen at 80?C.
The medium is thawed and dispensed as required.
If prepared from components:
HEPES (Sigma H-3375)……………………………..5.64 g
Neopeptone……………..……………………………4.7 g
Sodium citrate………………………………………...0.7 g
Glucose………………………………………………..5.64 g
NaHCO3………………………………………………2.0 g
TC-Yeastolate (BD 255772)………………………...2.0 g
Sodium pyruvate……………………………………..0.75 g
N-acetylglucosamine………………………………...0.37 g
Bovine Serum Albumin, Fraction V………………...47.0 g
CMRL 1066, 10X (w/o Glutamine or NaHCO3)…...100.0 ml
Rabbit Serum (heat inactivated)…………………….60.0 ml
DI Water………………………………………………..840 ml
Dissolve ingredients up to and including bovine serum albumin one at a time in distilled water. Adjust to pH 7.5 with NaOH and filter-sterilize.
Aseptically add CMRL 1066 and rabbit serum. Mix well and aseptically dispenses into appropriate vessel. Final pH of complete medium should be 7.5 - 7.6.