ATCC medium: 1981 M-R2A medium
NH4Cl ........................0.8 g
KH2PO4 .......................0.25 g
K2HPO4 .......................0.4 g
KNO3 .........................0.505 g
CaCl2 . 2H2O ................15.0 mg
MgCl2 . 6H2O ................20.0 mg
FeSO4 . 7H2O .................7.0 mg
Na2SO4 .......................5.0 mg
MnCl2 . 4H2O .................5.0 mg
H3BO3 ........................0.5 mg
ZnCl2 ........................0.5 mg
CoCl2 . 6H2O .................0.5 mg
NiSO4 . 6H2O .................0.5 mg
CuCl2 . 2H2O .................0.3 mg
Na2MoO4 . 2H2O...............10.0 mcg
Yeast extract................0.5 g
Peptone......................0.5 g
Casamino acids...............0.5 g
Dextrose.....................0.5 g
Soluble starch...............0.5 g
Sodium pyruvate..............0.5 g
Agar........................15.0 g
Distilled water..............1.0 L
Adjust medium for final pH 7.0. Autoclave at 121C for 15 minutes.