ATCC medium: 2382 Hagerdahl medium
Macro Salts 10X (per liter):
NaCl........................15.0 g
(NH4)2SO4....................31.0 g
Na2HPO4* ....................91.0 g
KH2PO4 .......................9.0 g
*Na2HPO4 . 7H2O, 172.0 g, may be used.
Adjust pH to 7.4 +/- 0.2.
Microsalts 50X (per 500 ml): EDTA.........................1.25 g
MgSO4 . 7H2O..................5.0 g
ZnSO4 . 7H2O..................0.2 g
FeSO4 . 7H2O..................0.5 g
MnSO4 . H2O...................0.38 g
CaCl2 . 2H2O..................0.66 g
Carbon Source (choose one):
Cellobiose*..................5.0 g/L
Glucose......................5.0 g/L
Xylan........................7.5-10.0 g/L
*(The organism prefers cellobiose.) Additions:
Biotin.......................1.0 mg/L
Thiamine ....................1.0 mg/L
Yeast extract**..............0.2 g/L
**(optional-not at all necessary)
Autoclave 10X Macro Salts and solid carbon source together. Cool and add sterile Microsalts to 1X, Soluble carbon source and “Additions”.