ATCC medium: 1294 Medium A
Yeast extract.......................................10.0 g
D-Glucose...........................................20.0 g
Biotin (filter-sterilized)...........................1.0 mg
Calcium D-(+)-pantothenate (filter-sterilized).......1.0 mg
Agar................................................20.0 g
Distilled water......................................1.0 L
Combine all ingredients except Biotin and Calcium D-(+)-pantothenate, adjust pH to 7.3,
autoclave at 121C for 15 minutes, then aseptically add the filter-sterilized components.