ATCC Medium: 43 Clostridium Medium
K2HPO4…………………………………..1.75 g
CaCl2 x 2H2O…………………………….0.01 g
MgSO4 x 7H2O…………………………...0.2 g
FeSO4 x 7H2O……………………………0.01 g
L-ArginineHCl………………….…………2.0 g
L-LysineHCl………………………………2.0 g
Yeast Extract……………………………...5.0 g
Sodium Formate………..….……………..2.0 g
NH4Cl………………………………………2.0 g
Methylene Blue (1mg/ml solution)….…..2.0 ml
Na2S x 9H2O Solution (see below)…….50 ml
Agar…………………………………..……15 g
Tap water……………………….…………950 ml
Autoclave at 121oC. Na2S x 9H2O Solution
Na2S x 9H2O………………………………0.3 g
DI Water……………………………………50 ml
Dispense anaerobically under 100% N2.