ATCC medium: 655 Thermobacterium medium
Yeast extract......................1.0 g
Tryptone (BD 211705)...............1.0 g
(NH4)2SO4...........................1.3 g
MgSO4 . 7H2O .......................0.247 g
KH2PO4 .............................0.28 g
CaCl2 . 2H2O .......................0.074 g
FeCl3 . 6H2O .......................0.019 g
Salt Solution (see below)..........1.0 ml
Agar..............................20.0 g
Distilled water....................1.0 L
Adjust pH to 8.5. Dispense in large screw-capped tubes, 11-12 ml/tube. Agar: Short slants, long butts.
Autoclave at 121C for 15 minutes.
Salt Solution:
MnCl2 . 4H2O .......................1.8 g
Na2B4O7 . 10H2O .....................4.4 g
ZnSO4 . 7H2O .......................0.22 g
CuCl2 . H2O ........................0.05 g
Na2MoO4 . 2H2O......................0.03 g
VOSO4 . 2H2O .......................0.03 g
Distilled water....................1.0 L
Adjust pH to 2.0 with H2SO4.