ATCC medium: 1473 LPBM acido-thermophile medium
NH4Cl .................................... 1.0 g
KH2PO4 ................................... 1.0 g
Na2HPO4 . 7H2O............................ 0.1 g
MgSO4 . 7H2O ............................. 0.2 g
CaCl2 . 2H2O ............................. 0.02 g
Yeast extract............................ 1.0 g
Sigmacell alpha Type 50 (Sigma S5504).... 5.0 g
Cellobiose............................... 0.5 g
Agar.................................... 20.0 g
Distilled water.......................... 1.0 L
Adjust medium to pH 5.2 with H3PO4 prior to addition of carbon sources. Autoclave at 121C for 15 minutes.