ATCC medium: 1767 Anaerobic modified MRS
Proteose Peptone No. 3 (BD 211693)......10.0 g
Yeast extract............................5.0 g
Beef extract.............................2.0 g
Glucose.................................10.0 g
K2HPO4 ...................................2.0 g
NaCl.....................................5.0 g
MgSO4 . 7H2O .............................0.2 g
MnSO4 . H2O ..............................0.05 g
Tween 80.................................1.0 ml
Salts Solution (see below)..............40.0 ml
L-Cysteine . HCl.........................0.5 g
Resazurin................................1.0 mg
Agar....................................10.0 g
Distilled water........................960.0 ml
Prepare medium anaerobically under 80% N2, 20% CO2. Adjust for final pH 7.0. Autoclave at 121C for 15 minutes.
Salts Solution:
CaCl2 (anhydrous) ............0.2 g
MgSO4 ........................0.2 g
K2HPO4 .......................1.0 g
KH2PO4 .......................1.0 g
NaHCO3 ......................10.0 g
NaCl.........................2.0 g
Distilled water..............1.0 L
Dissolve CaCl2 and MgSO4 in 300 ml of distilled water. Add 500 ml water and the remaining salts while swirling slowly. Add 200 ml of distilled water, mix, and store at 4C.