ATCC medium: 247 PPLO broth without CV (pH 7.8) with horse serum (not inactivated) and yeast extract
PPLO Broth w/o CV (Mycoplasma Broth) (BD 255420, pH 7.8)........ 21.0 g
Horse Serum (not inactivated).................................. 200.0 ml
Fresh Baker’s Yeast Extract (GIBCO 18180)...................... 100.0 ml
Distilled water................................................ 700.0 ml
Aseptically add the horse serum and GIBCO 18180 to a sterile basal medium containing PPLO broth and water.