ATCC medium: 1106 PPLO broth with bovine serum
PPLO Broth Base w/o CV (Mycoplasma Broth Base) (BD 211458)......21.0 g
Phenol red solution, 1% stock solution...........................2.0 ml
Deionized water................................................865.0 ml
Adjust pH to 7.5. Autoclave. Aseptically add:
Glucose (20% solution)*.........................................25.0 ml
Bovine serum*...................................................10.0 ml
Yeast Extract Solution (GIBCO 18180)...........................100.0 ml
*Filter-sterilize.