ATCC medium: 1057 Methylococcus medium
Sodium-Potassium Phosphate Buffer, pH 7.10 (see below).. 6.5 ml
Sodium nitrate solution (20%).......................... 10.0 ml
L-F Salts Solution (see below)......................... 10.0 ml
Distilled water....................................... 973.5 ml
Cultivate in an atmosphere of 50% methane, 50% air.
Sodium-Potassium Phosphate Buffer:
KH2PO4 ....................................136.0 g
NaOH......................................28.8 g
Distilled water...........................1.0 L
L-F Salts Solution:
10% (w/v) MgSO4 . 7H2O solution ...........200.0 ml
10% (w/v) CaCl2 . 2H2O solution ...........20.0 ml
1% (w/v) ZnSO4 . 7H2O solution ............4.9 ml
1% (w/v) CuSO4 . 5H2O solution ............0.2 ml
1% (w/v) H3BO3 solution ...................0.6 ml
1% (w/v) MnSO4 . H2O solution .............0.27 ml
10% (w/v) FeSO4 solution ..................10.0 ml
Distilled water to........................1.0 L
The FeSO4 solution is either filter-sterilized or acidified with 2%
(v/v) HCl prior to autoclaving. For solid medium, add 8.5 g/L of Agar,
Noble (BD 214230). For basal medium, grow culture in an atmosphere of
50% methane, 50% air.