ATCC medium: 1301 Marine peptone - succinate - salts medium (PSS)
Peptone, Bacto (BD 211677)........ 10.0 g
Succinic acid...................... 1.0 g
(NH4)2SO4........................... 1.0 g
MgSO4 . 7H2O ....................... 1.0 g
FeCl3 . 6H2O ....................... 2.0 mg
MnSO4 . H2O ........................ 2.0 mg
Synthetic Seawater (see below)..... 1.0 L
Synthetic Seawater:
NaCl ..............................27.5 g
MgCl2 .............................. 5.0 g
MgSO4 . 7H2O ....................... 2.0 g
CaCl2 .............................. 0.5 g
KCl................................ 1.0 g
FeSO4 .............................. 1.0 mg
Distilled water.................... 1.0 L
Adjust pH to 6.8 with KOH. Autoclave at 121C for 15 minutes.