ATCC medium: 1986 V8 juice seawater agar
Unsalted V8 Juice............................ 200.0 ml
CaCO3 .......................................... 3.0 g
Agar.......................................... 15.0 g
Artificial Seawater (2% NaCl) (see below).... 800.0 ml
Adjust medium for final pH 7.0. Autoclave at 121C for 15 minutes.
Artificial Seawater (2% NaCl):
NaCl........................ 20.0g
MgCl2 . 6H2O ................. 5.38 g
MgSO4 . 7H2O ................. 6.78 g
KCl.......................... 0.72 g
NaHCO3 ....................... 0.2 g
CaCl2 . 2H2O ................. 1.4 g
Distilled water.............. 1.0 L