ATCC medium: 1676 Modified Van Niel's medium
MgSO4 ................................................0.1 g
EDTA.................................................2.0 mg
Yeast extract.......................................10.0 g
Trace Elements (see below)..........................10.0 ml
4% K2HPO4 (add aseptically after sterilization) ......2.5 ml
Distilled water....................................985.0 ml
Adjust medium for final pH 7.1. Autoclave at 121C for 15 minutes.
Trace Elements:
Ferric ammonium citrate............0.2 g
CaCl2 . 2H2O .......................0.3 g
Distilled water..................100.0 ml
Filter-sterilize and add aseptically to basal medium.