ATCC medium: 1162 Methanol-urea mineral
salts medium KH2PO4 .........................................1.36 g
Na2HPO4 ........................................2.13 g
(NH4)2SO4.......................................0.5 g
MgSO4 . 7H2O ...................................0.2 g
CaCl2 . 2H2O ...................................0.01 g
FeSO4 . 7H2O ...................................5.0 mg
MnSO4 . 5H2O ...................................2.5 mg
Na2MoO4 . 2H2O..................................2.5 mg
Distilled water................................1.0 L
Autoclave at 121C for 15 minutes.
To this autoclaved base add: Methanol (filter-sterilized)...................5.0 ml Urea (20% solution, filter-sterilized)........30.0 ml